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two-photon imaging system ultima  (Bruker Corporation)


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    Bruker Corporation two-photon imaging system ultima
    Two Photon Imaging System Ultima, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/two-photon+imaging/two+photon+microscope+ultima+iv/pm40614201-168-34-38
    Average 90 stars, based on 1 article reviews
    two-photon imaging system ultima - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Fluorescence:

    Article Title: Selective glycinergic input from vGluT3 amacrine cells confers a suppressed‐by‐contrast trigger feature in a subtype of M1 ipRGCs in the mouse retina
    Article Snippet: .. Z-series stacks of two-photon images of fluorescence labelling and whole-cell patch clamped cells (filled with Alexa Fluor 594) in the whole-mount retina were taken under two-photon imaging and processed with Prairie View (Bruker Nano, Inc.), ImageJ (NIH, Bethesda, MD, USA) and custom-made programs using Matlab 2018a (MathWorks).Dendritic density ofM1 cellswas computed as the number of pixels occupied by dendrites per annulus region (25 μm-thick, radius = 25 μm, centred on the soma) divided by the total number of pixels in the annulus area. ..

    Imaging:

    Article Title: Selective glycinergic input from vGluT3 amacrine cells confers a suppressed‐by‐contrast trigger feature in a subtype of M1 ipRGCs in the mouse retina
    Article Snippet: .. Z-series stacks of two-photon images of fluorescence labelling and whole-cell patch clamped cells (filled with Alexa Fluor 594) in the whole-mount retina were taken under two-photon imaging and processed with Prairie View (Bruker Nano, Inc.), ImageJ (NIH, Bethesda, MD, USA) and custom-made programs using Matlab 2018a (MathWorks).Dendritic density ofM1 cellswas computed as the number of pixels occupied by dendrites per annulus region (25 μm-thick, radius = 25 μm, centred on the soma) divided by the total number of pixels in the annulus area. ..



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    ( A ) Schematic of excitatory cortical projection neuron subtypes (left). Representative coronal sections of ACtx showing GCaMP8s expression (green) and 4′,6-diamidino-2-phenylindole (DAPI)–labeled (blue) nuclei (right). The transgenic and viral strategies noted in (B) were used to target L2/3, L5 IT, L5 ET, or CT neurons. WM, white matter. Scale bar, 250 μm. ( B ) Overview of the experimental design. Mice passively listen to pure tone frequencies, <t>while</t> <t>two-photon</t> calcium imaging and pupillometry were performed simultaneously. Cell-type–specific targeting strategies (top) used a combination of viral and/or transgenic methods. WT, wild type. Exemplar two-photon fields of view (FOVs) of each target ACtx cell subpopulation (right). Scale bar, 250 μm. ( C ) Exemplar 5-min recording showing pupil diameter (top; black), deconvolved neural activity from a single ET neuron (middle; orange), and sound onset times (bottom). Pupil size is expressed as a percentage of maximum diameter; neural activity is plotted in SDs ( z -scored) from baseline. ( D ) Histogram of trialwise pupil states across all mice. Counts refer to number of trials. Median pupil size of 62% is denoted by the yellow dashed line.
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    ( A ) Schematic of excitatory cortical projection neuron subtypes (left). Representative coronal sections of ACtx showing GCaMP8s expression (green) and 4′,6-diamidino-2-phenylindole (DAPI)–labeled (blue) nuclei (right). The transgenic and viral strategies noted in (B) were used to target L2/3, L5 IT, L5 ET, or CT neurons. WM, white matter. Scale bar, 250 μm. ( B ) Overview of the experimental design. Mice passively listen to pure tone frequencies, <t>while</t> <t>two-photon</t> calcium imaging and pupillometry were performed simultaneously. Cell-type–specific targeting strategies (top) used a combination of viral and/or transgenic methods. WT, wild type. Exemplar two-photon fields of view (FOVs) of each target ACtx cell subpopulation (right). Scale bar, 250 μm. ( C ) Exemplar 5-min recording showing pupil diameter (top; black), deconvolved neural activity from a single ET neuron (middle; orange), and sound onset times (bottom). Pupil size is expressed as a percentage of maximum diameter; neural activity is plotted in SDs ( z -scored) from baseline. ( D ) Histogram of trialwise pupil states across all mice. Counts refer to number of trials. Median pupil size of 62% is denoted by the yellow dashed line.
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    Image Search Results


    ( A ) Schematic of excitatory cortical projection neuron subtypes (left). Representative coronal sections of ACtx showing GCaMP8s expression (green) and 4′,6-diamidino-2-phenylindole (DAPI)–labeled (blue) nuclei (right). The transgenic and viral strategies noted in (B) were used to target L2/3, L5 IT, L5 ET, or CT neurons. WM, white matter. Scale bar, 250 μm. ( B ) Overview of the experimental design. Mice passively listen to pure tone frequencies, while two-photon calcium imaging and pupillometry were performed simultaneously. Cell-type–specific targeting strategies (top) used a combination of viral and/or transgenic methods. WT, wild type. Exemplar two-photon fields of view (FOVs) of each target ACtx cell subpopulation (right). Scale bar, 250 μm. ( C ) Exemplar 5-min recording showing pupil diameter (top; black), deconvolved neural activity from a single ET neuron (middle; orange), and sound onset times (bottom). Pupil size is expressed as a percentage of maximum diameter; neural activity is plotted in SDs ( z -scored) from baseline. ( D ) Histogram of trialwise pupil states across all mice. Counts refer to number of trials. Median pupil size of 62% is denoted by the yellow dashed line.

    Journal: Science Advances

    Article Title: Pupil-linked arousal heterogeneously modulates cell-type–specific sensory processing

    doi: 10.1126/sciadv.adz6495

    Figure Lengend Snippet: ( A ) Schematic of excitatory cortical projection neuron subtypes (left). Representative coronal sections of ACtx showing GCaMP8s expression (green) and 4′,6-diamidino-2-phenylindole (DAPI)–labeled (blue) nuclei (right). The transgenic and viral strategies noted in (B) were used to target L2/3, L5 IT, L5 ET, or CT neurons. WM, white matter. Scale bar, 250 μm. ( B ) Overview of the experimental design. Mice passively listen to pure tone frequencies, while two-photon calcium imaging and pupillometry were performed simultaneously. Cell-type–specific targeting strategies (top) used a combination of viral and/or transgenic methods. WT, wild type. Exemplar two-photon fields of view (FOVs) of each target ACtx cell subpopulation (right). Scale bar, 250 μm. ( C ) Exemplar 5-min recording showing pupil diameter (top; black), deconvolved neural activity from a single ET neuron (middle; orange), and sound onset times (bottom). Pupil size is expressed as a percentage of maximum diameter; neural activity is plotted in SDs ( z -scored) from baseline. ( D ) Histogram of trialwise pupil states across all mice. Counts refer to number of trials. Median pupil size of 62% is denoted by the yellow dashed line.

    Article Snippet: All two-photon imaging (Bergamo, Thorlabs) was of the right ACtx.

    Techniques: Expressing, Labeling, Transgenic Assay, Imaging, Activity Assay